Replication protein A1

Protein-coding gene in the species Homo sapiens

RPA1

Available structures

PDB

Ortholog search: PDBe RCSB

List of PDB id codes
1EWI, 1FGU, 1JMC, 1L1O, 2B29, 2B3G, 4IJH, 4IJL, 4IPC, 4IPD, 4IPG, 4IPH, 4LUO, 4LUV, 4LUZ, 4LW1, 4LWC, 4NB3, 4O0A, 4R4C, 4R4I, 4R4O, 4R4Q, 4R4T, 5E7N, 5EAY

Identifiers

Aliases

RPA1, HSSB, MST075, REPA1, RF-A, RP-A, RPA70, Replication protein A1, PFBMFT6

External IDs

OMIM: 179835; MGI: 1915525; HomoloGene: 2208; GeneCards: RPA1; OMA:RPA1 - orthologs

Gene location (Human)

Chr.	Chromosome 17 (human)^[1]

Band	17p13.3	Start	1,829,702 bp^[1]
Band	17p13.3	End	1,900,082 bp^[1]

Gene location (Mouse)

Chr.	Chromosome 11 (mouse)^[2]

Band	11 B5\|11 45.79 cM	Start	75,188,992 bp^[2]
Band	11 B5\|11 45.79 cM	End	75,239,150 bp^[2]

RNA expression pattern

Bgee

Human

Mouse (ortholog)

Top expressed in

secondary oocyte

ventricular zone

ganglionic eminence

parietal pleura

visceral pleura

germinal epithelium

gingival epithelium

trabecular bone

tibia

parotid gland

Top expressed in

fetal liver hematopoietic progenitor cell

retinal pigment epithelium

ciliary body

primitive streak

vestibular membrane of cochlear duct

iris

Epithelium of choroid plexus

abdominal wall

endothelial cell of lymphatic vessel

Paneth cell

More reference expression data

BioGPS

n/a

Gene ontology
Molecular function	DNA binding metal ion binding single-stranded DNA binding damaged DNA binding protein binding nucleic acid binding G-rich strand telomeric DNA binding single-stranded telomeric DNA binding sequence-specific DNA binding
Cellular component	PML body nucleoplasm DNA replication factor A complex nucleus site of DNA damage
Biological process	DNA-dependent DNA replication nucleotide-excision repair, DNA gap filling DNA recombination interstrand cross-link repair error-free translesion synthesis G1 phase error-prone translesion synthesis cellular response to DNA damage stimulus DNA replication regulation of cellular response to heat DNA mismatch repair translesion synthesis transcription-coupled nucleotide-excision repair nucleotide-excision repair, DNA incision nucleotide-excision repair, DNA incision, 3'-to lesion nucleotide-excision repair, DNA incision, 5'-to lesion nucleotide-excision repair, preincision complex stabilization nucleotide-excision repair DNA repair base-excision repair regulation of signal transduction by p53 class mediator double-strand break repair via homologous recombination nucleotide-excision repair, preincision complex assembly protein localization to chromosome telomere maintenance telomere maintenance via semi-conservative replication G1/S transition of mitotic cell cycle DNA unwinding involved in DNA replication telomere maintenance via telomerase meiosis
Sources:Amigo / QuickGO

Orthologs

Species

Human

Mouse

Entrez


6117


68275

Ensembl


ENSG00000132383


ENSMUSG00000000751

UniProt


P27694


Q8VEE4

RefSeq (mRNA)


NM_002945 NM_001355120 NM_001355121


NM_001164223 NM_026653

RefSeq (protein)


NP_002936 NP_001342049 NP_001342050


NP_001157695 NP_080929

Location (UCSC)

Chr 17: 1.83 – 1.9 Mb

Chr 11: 75.19 – 75.24 Mb

PubMed search

^[3]

^[4]

Wikidata

View/Edit Human

View/Edit Mouse

Replication protein A 70 kDa DNA-binding subunit is a protein that in humans is encoded by the RPA1 gene.^[5]

Interactions

Replication protein A1 has been shown to interact with:

References

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000132383 – Ensembl, May 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000000751 – Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Umbricht CB, Erdile LF, Jabs EW, Kelly TJ (April 1993). "Cloning, overexpression, and genomic mapping of the 14-kDa subunit of human replication protein A". J Biol Chem. 268 (9): 6131–8. doi:10.1016/S0021-9258(18)53229-4. PMID 8454588.
^ Wong JM, Ionescu D, Ingles CJ (January 2003). "Interaction between BRCA2 and replication protein A is compromised by a cancer-predisposing mutation in BRCA2". Oncogene. 22 (1): 28–33. doi:10.1038/sj.onc.1206071. PMID 12527904.
^ Brosh RM, Li JL, Kenny MK, Karow JK, Cooper MP, Kureekattil RP, Hickson ID, Bohr VA (August 2000). "Replication protein A physically interacts with the Bloom's syndrome protein and stimulates its helicase activity". J. Biol. Chem. 275 (31): 23500–8. doi:10.1074/jbc.M001557200. hdl:10026.1/10318. PMID 10825162.
^ Opresko PL, von Kobbe C, Laine JP, Harrigan J, Hickson ID, Bohr VA (October 2002). "Telomere-binding protein TRF2 binds to and stimulates the Werner and Bloom syndrome helicases". J. Biol. Chem. 277 (43): 41110–9. doi:10.1074/jbc.M205396200. PMID 12181313.
^ Moens PB, Kolas NK, Tarsounas M, Marcon E, Cohen PE, Spyropoulos B (April 2002). "The time course and chromosomal localization of recombination-related proteins at meiosis in the mouse are compatible with models that can resolve the early DNA-DNA interactions without reciprocal recombination". J. Cell Sci. 115 (Pt 8): 1611–22. doi:10.1242/jcs.115.8.1611. PMID 11950880.
^ ^a ^b ^c ^d ^e ^f Kneissl M, Pütter V, Szalay AA, Grummt F (March 2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". J. Mol. Biol. 327 (1): 111–28. doi:10.1016/s0022-2836(03)00079-2. PMID 12614612.
^ Parker A, Gu Y, Mahoney W, Lee SH, Singh KK, Lu AL (February 2001). "Human homolog of the MutY repair protein (hMYH) physically interacts with proteins involved in long patch DNA base excision repair". J. Biol. Chem. 276 (8): 5547–55. doi:10.1074/jbc.M008463200. PMID 11092888.
^ Romanova LY, Willers H, Blagosklonny MV, Powell SN (Dec 2004). "The interaction of p53 with replication protein A mediates suppression of homologous recombination". Oncogene. 23 (56): 9025–33. doi:10.1038/sj.onc.1207982. PMID 15489903. S2CID 23482723.
^ Riva F, Zuco V, Vink AA, Supino R, Prosperi E (Dec 2001). "UV-induced DNA incision and proliferating cell nuclear antigen recruitment to repair sites occur independently of p53-replication protein A interaction in p53 wild type and mutant ovarian carcinoma cells". Carcinogenesis. 22 (12): 1971–8. doi:10.1093/carcin/22.12.1971. PMID 11751427.
^ Shao RG, Cao CX, Zhang H, Kohn KW, Wold MS, Pommier Y (March 1999). "Replication-mediated DNA damage by camptothecin induces phosphorylation of RPA by DNA-dependent protein kinase and dissociates RPA:DNA-PK complexes". EMBO J. 18 (5): 1397–406. doi:10.1093/emboj/18.5.1397. PMC 1171229. PMID 10064605.
^ ^a ^b Bochkareva E, Korolev S, Lees-Miller SP, Bochkarev A (April 2002). "Structure of the RPA trimerization core and its role in the multistep DNA-binding mechanism of RPA". EMBO J. 21 (7): 1855–63. doi:10.1093/emboj/21.7.1855. PMC 125950. PMID 11927569.
^ Bochkareva E, Frappier L, Edwards AM, Bochkarev A (February 1998). "The RPA32 subunit of human replication protein A contains a single-stranded DNA-binding domain". J. Biol. Chem. 273 (7): 3932–6. doi:10.1074/jbc.273.7.3932. PMID 9461578.
^ Unsal-Kaçmaz K, Chastain PD, Qu PP, Minoo P, Cordeiro-Stone M, Sancar A, Kaufmann WK (April 2007). "The human Tim/Tipin complex coordinates an Intra-S checkpoint response to UV that slows replication fork displacement". Mol. Cell. Biol. 27 (8): 3131–42. doi:10.1128/MCB.02190-06. PMC 1899931. PMID 17296725.
^ Yoo E, Kim BU, Lee SY, Cho CH, Chung JH, Lee CH (August 2005). "53BP1 is associated with replication protein A and is required for RPA2 hyperphosphorylation following DNA damage". Oncogene. 24 (35): 5423–30. doi:10.1038/sj.onc.1208710. PMID 15856006.
^ Li L, Lu X, Peterson CA, Legerski RJ (October 1995). "An interaction between the DNA repair factor XPA and replication protein A appears essential for nucleotide excision repair". Mol. Cell. Biol. 15 (10): 5396–402. doi:10.1128/mcb.15.10.5396. PMC 230789. PMID 7565690.

Iftode C, Daniely Y, Borowiec JA (1999). "Replication protein A (RPA): the eukaryotic SSB". Crit. Rev. Biochem. Mol. Biol. 34 (3): 141–80. doi:10.1080/10409239991209255. PMID 10473346.
Binz SK, Sheehan AM, Wold MS (2005). "Replication protein A phosphorylation and the cellular response to DNA damage". DNA Repair (Amst.). 3 (8–9): 1015–24. doi:10.1016/j.dnarep.2004.03.028. PMID 15279788.
Dornreiter I, Erdile LF, Gilbert IU, von Winkler D, Kelly TJ, Fanning E (1992). "Interaction of DNA polymerase alpha-primase with cellular replication protein A and SV40 T antigen". EMBO J. 11 (2): 769–76. doi:10.1002/j.1460-2075.1992.tb05110.x. PMC 556510. PMID 1311258.
Erdile LF, Heyer WD, Kolodner R, Kelly TJ (1991). "Characterization of a cDNA encoding the 70-kDa single-stranded DNA-binding subunit of human replication protein A and the role of the protein in DNA replication". J. Biol. Chem. 266 (18): 12090–8. doi:10.1016/S0021-9258(18)99069-1. PMID 2050703.
Li L, Lu X, Peterson CA, Legerski RJ (1995). "An interaction between the DNA repair factor XPA and replication protein A appears essential for nucleotide excision repair". Mol. Cell. Biol. 15 (10): 5396–402. doi:10.1128/mcb.15.10.5396. PMC 230789. PMID 7565690.
Keshav KF, Chen C, Dutta A (1995). "Rpa4, a homolog of the 34-kilodalton subunit of the replication protein A complex". Mol. Cell. Biol. 15 (6): 3119–28. doi:10.1128/mcb.15.6.3119. PMC 230543. PMID 7760808.
Umbricht CB, Griffin CA, Hawkins AL, Grzeschik KH, O'Connell P, Leach R, Green ED, Kelly TJ (1994). "High-resolution genomic mapping of the three human replication protein A genes (RPA1, RPA2, and RPA3)". Genomics. 20 (2): 249–57. doi:10.1006/geno.1994.1161. PMID 8020972.
Gomes XV, Wold MS (1996). "Functional domains of the 70-kilodalton subunit of human replication protein A.". Biochemistry. 35 (32): 10558–68. doi:10.1021/bi9607517. PMID 8756712.
Bochkarev A, Pfuetzner RA, Edwards AM, Frappier L (1997). "Structure of the single-stranded-DNA-binding domain of replication protein A bound to DNA". Nature. 385 (6612): 176–81. Bibcode:1997Natur.385..176B. doi:10.1038/385176a0. PMID 8990123. S2CID 4288187.
Amacker M, Hottiger M, Mossi R, Hübscher U (1997). "HIV-1 nucleocapsid protein and replication protein A influence the strand displacement DNA synthesis of lentiviral reverse transcriptase". AIDS. 11 (4): 534–6. PMID 9084803.
Cujec TP, Cho H, Maldonado E, Meyer J, Reinberg D, Peterlin BM (1997). "The human immunodeficiency virus transactivator Tat interacts with the RNA polymerase II holoenzyme". Mol. Cell. Biol. 17 (4): 1817–23. doi:10.1128/MCB.17.4.1817. PMC 232028. PMID 9121429.
Loor G, Zhang SJ, Zhang P, Toomey NL, Lee MY (1998). "Identification of DNA replication and cell cycle proteins that interact with PCNA". Nucleic Acids Res. 25 (24): 5041–6. doi:10.1093/nar/25.24.5041. PMC 147130. PMID 9396813.
Bochkareva E, Frappier L, Edwards AM, Bochkarev A (1998). "The RPA32 subunit of human replication protein A contains a single-stranded DNA-binding domain". J. Biol. Chem. 273 (7): 3932–6. doi:10.1074/jbc.273.7.3932. PMID 9461578.
Golub EI, Gupta RC, Haaf T, Wold MS, Radding CM (1999). "Interaction of human rad51 recombination protein with single-stranded DNA binding protein, RPA". Nucleic Acids Res. 26 (23): 5388–93. doi:10.1093/nar/26.23.5388. PMC 148005. PMID 9826763.
Shao RG, Cao CX, Zhang H, Kohn KW, Wold MS, Pommier Y (1999). "Replication-mediated DNA damage by camptothecin induces phosphorylation of RPA by DNA-dependent protein kinase and dissociates RPA:DNA-PK complexes". EMBO J. 18 (5): 1397–406. doi:10.1093/emboj/18.5.1397. PMC 1171229. PMID 10064605.
Brosh RM, Orren DK, Nehlin JO, Ravn PH, Kenny MK, Machwe A, Bohr VA (1999). "Functional and physical interaction between WRN helicase and human replication protein A." J. Biol. Chem. 274 (26): 18341–50. doi:10.1074/jbc.274.26.18341. PMID 10373438.
Kim ST, Lim DS, Canman CE, Kastan MB (2000). "Substrate specificities and identification of putative substrates of ATM kinase family members". J. Biol. Chem. 274 (53): 37538–43. doi:10.1074/jbc.274.53.37538. PMID 10608806.
Brosh RM, Li JL, Kenny MK, Karow JK, Cooper MP, Kureekattil RP, Hickson ID, Bohr VA (2000). "Replication protein A physically interacts with the Bloom's syndrome protein and stimulates its helicase activity". J. Biol. Chem. 275 (31): 23500–8. doi:10.1074/jbc.M001557200. hdl:10026.1/10318. PMID 10825162.

PDB gallery

1ewi: HUMAN REPLICATION PROTEIN A: GLOBAL FOLD OF THE N-TERMINAL RPA-70 DOMAIN REVEALS A BASIC CLEFT AND FLEXIBLE C-TERMINAL LINKER
1fgu: SSDNA-BINDING DOMAIN OF THE LARGE SUBUNIT OF REPLICATION PROTEIN A
1jmc: SINGLE STRANDED DNA-BINDING DOMAIN OF HUMAN REPLICATION PROTEIN A BOUND TO SINGLE STRANDED DNA, RPA70 SUBUNIT, RESIDUES 183-420
1l1o: Structure of the human Replication Protein A (RPA) trimerization core
2b29: N-terminal domain of the RPA70 subunit of human replication protein A.
2b3g: p53N (fragment 33-60) bound to RPA70N

DNA replication (comparing prokaryotic to eukaryotic)

Initiation

Prokaryotic (initiation)	Pre-replication complex dnaC Helicase dnaA dnaB T7 Primase dnaG
Eukaryotic (preparation in G1 phase)	Pre-replication complex Origin recognition complex ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 Cdc6 Cdt1 Minichromosome maintenance MCM2 MCM3 MCM4 MCM5 MCM6 MCM7 Licensing factor Autonomously replicating sequence Single-strand binding protein SSBP2 SSBP3 SSBP4 RNase H RNASEH1 RNASEH2A Helicase: HFM1 Primase: PRIM1 PRIM2
Both	Origin of replication/Ori/Replicon Replication fork Lagging and leading strands Okazaki fragments Primer

Replication

Prokaryotic (elongation)	DNA polymerase III holoenzyme dnaC dnaE dnaH dnaN dnaQ dnaT dnaX holA holB holC holD holE Replisome DNA ligase DNA clamp Topoisomerase DNA gyrase Prokaryotic DNA polymerase: DNA polymerase I Klenow fragment
Eukaryotic (synthesis in S phase)	Replication factor C RFC1 Flap endonuclease FEN1 Topoisomerase Replication protein A RPA1 Eukaryotic DNA polymerase: alpha POLA1 POLA2 PRIM1 PRIM2 delta POLD1 POLD2 POLD3 POLD4 epsilon POLE POLE2 POLE3 POLE4 DNA clamp PCNA Control of chromosome duplication
Both	Movement: Processivity DNA ligase